Impact of changes in the amino acid sequence of the "a" determinant on the diagnostic capacities of test systems for the detection of HBsAg


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The diagnostic capacities of 4 commercial test systems were comparatively estimated for the detection of HBsAg, by applying a panel of samples with the established amino acid sequence of the "a" determinant of HBsAg. The Roche Elecsys HBsAg test system demonstrated the highest sensitivity - the maximum HBsAg concentrations were found in 19 of 31 cases. Escape mutations in the major hydrophilic region (MHR) of HBsAG (P120S, M133T) were responsible for differences in the sensitivity of 4 test systems by 10- to 40-fold. There were also samples that showed differences in the diagnostic capacities of the test systems to detect HBsAg, but without amino acid replacements in the area of the "a" determinant, which seems to be associated with amino acid replacements in other regions of HBsAg.

References

  1. Баженов А. И., Годков М. А., Лапина Н. Е. и др. Сравнительная оценка способности коммерческих иммунодиагностикумов выявлять HbsAg "ускользающих" мутантов вируса гепатита В (ВГВ) // Новые информационные технологии в медицине, биологии, фармакологии и экологии. - Гурзуф, 2007. - С. 168-169.
  2. Забелин Н. Н. Генетическая вариабельность вируса гепатита В по preS1/preS2/S-генам у пациентов с хронической HB-вирусной инфекцией: Автореф. дис. ... канд. мед. наук. - М., 2009.
  3. Devesa M., Rodriguez C., Leon G. et al. Clade analysis and surface antigen polymorphism of hepatitis B virus American genotypes // J. Med. Virol. - 2004. - Vol. 72. - P. 377-384.
  4. Echevarria J. M., Avellon A. Hepatitis B virus genetic diversity // J. Med. Virol. - 2006. - Vol. 78. - P. S36-S42.
  5. He C., Nomura F., Itoga S. et al. Prevalence vaccine-induced escape mutants of hepatitis B virus in the adult population in China: a prospective study in 176 restaurant employees // J. Gastroenterol. Hepatol. - 2001. - Vol. 16. - P. 1373-1377.
  6. Hou J., Wang Z., Cheng J. et al. Prevalence of naturally occurring surface gene variants of hepatitis B virus nonimmunized surface antigen - negative Chinese carriers // J. Hepatol. - 2001. - Vol. 34. - P. 1027-1034.
  7. Ireland J. H., O'Donnell B., Basuni A. A. et al. Reactivity of 13 in vitro expressed hepatitis B surface antigen variants in 7 commercial diagnostic assays // J. Hepatol. - 2000. - Vol. 31. - P. 1176-1182.
  8. Moerman B., Moons V., Sommer H. et al. Evaluation of sensitivity for wild type and mutant forms of hepatitis B surface antigen by four commercial HbsAg assays // Clin. Lab. - 2004. - Vol. 50. - P. 159-162.
  9. Olinger C. M., Weber B., Otegbayo J. A. et al. Hepatitis B virus genotype E surface antigen detection with different immunoassays and diagnostic impact of mutations in the preS/S gene // Med. Microbiol. Immunol. - 2007. - Vol. 196. - P. 247-252.
  10. Oon C. J., Ning C. W., Shiuan K. et al. Identification of hepatitis B surface antigen variants with alterations outside the "a" determinant in immunized Singapore infants // J. Infect. Dis. - 1999. - Vol. 179. - P. 259-263.
  11. Szomor K. N., Dencs A., Toth G. et al. Variability of the PreS1/ PreS2/S regions of hepatitis B virus in Hungary // Arch. Virol. - 2007. - Vol. 152. - P. 697-704.
  12. Weber B., Dengler T., Berger A. et al. Evaluation of two new automated assays for hepatitis B virus antigen (HbsAg) detection: IMMULATE HbsAg and IMMULATE 2000 HbsAg // J. Clin. Microbiol. - 2003. - Vol. 41. - P. 135-143.
  13. Weber B. Genetic variability of the S gene of hepatitis B virus: clinical and diagnostic impact // J. Clin. Virol. - 2005. - Vol. 32. - P. 102-112.
  14. Weber B. Diagnostic impact of the genetic variability of the hepatitis B virus surface antigen gene // J. Med. Virol. - 2006. - Vol. 78. - P. S59-S65.
  15. Weinberger K. M., Bauer T., Bohm S. et al. High genetic variability of the group-specific a-determinant of hepatitis virus surface antigen (HbsAg) and the corresponding fragment of the viral polymerase in chronic virus carriers lacking detectable HbsAg in serum // J. Gen. Virol. - 2000. - Vol. 81. - P. 1165- 1174.

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Copyright (c) 2010 Zabelin N.N., Kuzina L.E., Kudryavtseva E.N., Zabotina E.E., Braginskiy D.M., Malyshev N.A., Kuzin S.N., Samokhvalov E.I., L'vov D.K., Zabelin N.N., Kuzina L.E., Kudryavtseva E.N., Zabotina E.E., Braginsky D.M., Malyshev N.A., Kuzin S.N., Samokhvalov E.I., Lvov D.K.

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