Comparison of different ELISAs for detection of antibodies to swine vesicular disease virus in sera from experimentally infected animals


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Abstract

The study has shown the efficiency of a competitive ELISA (C-ELISA) variant or an indirect ELISA (I-ELUSA) in the detection of antibodies to swine vesicular disease virus (SVDV) versus traditional assays, such as a microneutralization test, a blocking ELIDA test, and a the reference test Ceditest® SVDV (Cedi-Diagnostics B.V., Netherlands). Specific antibodies in the pig sera could be detected by C-ELISA on days 4-5 and by I-ELISA on day 6 after experimental SVDV infection. Specific antibodies were detected in a contact pig 11 days after the beginning of the experiment.

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Copyright (c) 2010 Lugovskaya N.N., Kharitonova E.N., Nikiforov V.V., Zhil'tsova M.V., Kremenchugskaya S.R., Borisov V.V., Lugovskaya N.N., Kharitonova E.N., Nikiforov V.V., Zhiltsova M.V., Kremenchugskaya S.R., Borisov V.V.

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