The influence of tetracycline on the infection properties of hepatitis-C virus and on the activity of mRNA cytokines in infected cultures of SW-13 and MT-4 cells


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Abstract

An experimental HCV-infection model was used in vitro to study the influence of tetracycline on the reproduction of the HCV-infection accompanied by a simultaneous analysis, by using the methods of reverse transcription and polymerase chain reaction (RT-PCR), of the mRNA activity, i.e. a-IFN, y-IFN, 1 p-IFN, IL-2, IL-4, IL-6, IL-6, IL-10, IL-12, IL-18 and TNF- a. The study was made with the SW-13 long-term cellular cultures (human adenocarcinoma cells) and MT-4 cell cultures (human lymphoblastoid cells) infected by HCV. An analysis of the obtained data is indicative, in both examined cellular cultures, of a differently-oriented induction-suppression reaction of cytokine mRNAs. Suppression of the activity of the investigated cytokine mRNAs, except for mRNAs IL-18 and TNF- a, was observed in the SW-13 cellular culture; while, as for the MT-4 cellular culture, the activity of all studied cytokine mRNAs was pointed out. The results testify to that tetracycline should be studied more actively in experimental infections caused by HCV in vivo.

References

  1. Дерябин П. Г., Исаева Е. И., Вязов С. О., Львов Д. К. Летальная инфекция новорожденных мышей, вызванная вирусом гепатита С // Вопр. вирусол. - 1997. - № 6. - С. 251- 253.
  2. Дерябин П. Г., Исаева Е. И., Львов Д. К. Антигенные и инфекционные свойства изолированных вариантов вируса гепатита С // Гепатиты В, С и Д. Проблемы диагностики, лечения и профилактики: Тез. докл. 3-й Всероссийской науч.-практ. конф. с международным участием. - М., 1999. - С. 62-63.
  3. Adrian M. Di Biseglie. Hepatitis С // Lancet. - 1998. - Vol.351.- P.351-355.
  4. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinum thyocyoanate-phenol-chloroform extraction // Anal. Biochem. - 1987. - Vol. 162. - P. 156-159.
  5. Clarke B. Another step forward in hepatitis С therapy // Lancet. - 2001. - Vol. 49. - Suppl. 1. - P. 1.
  6. Deryabin P. G., Lvov D. K., Isaeva E. I. Biological properties of cytopathogenic hepatitis С variants // 11-th International Congress of Virology. - Sydney, 1999. - Vol. 13. - P. 2.
  7. Gaede K. I., Mamat U., Schlaak M. et al. Analysis of differentially regulated mRNAs in monocytis cells induced by in vitro stimulation // J. Mol. Med. - 1999. - Vol.77, N 12. P. 847-852.
  8. Gelder С. M., Thomas P. S., Yates D. H. et al. Cytokine expression in normal, atopic, and asthmatic subject using the combination of sputum induction and the polimerase chain reaction // Thorax. - 1995. - Vol. 50. - P. 1033-1037.
  9. Gossen M., Bujuard H. Tight control of gene expression in mammalian cells by tetracycline responsive promoters // Proc. Natl. Acad. Sci. USA. - 1992. - Vol. 89. - P. 5547-5551.
  10. Lin Y., Zhang M., Barnes P. F. Chemokine production by a human alveolar epithelial cell line in response to Mycobacterium tuberculosis // Infect and Immun. - 1998. - Vol. 66, N 3. - P. 1121-1126.
  11. Lindsay K. L. Therapy of hepatitis C: overview // Hepatology. - 1997. - Vol. 26. - P. 71-77.
  12. Narovlansky A. N., Vershinina M. Yu., Amchenkova A. M. et al. The cytokine mRNAs in cell lines with different IFN-sensitivity // European Cytokine Network. Special issue 1CS/ISICR. - 2000. -Vol. 11. - P. 147.
  13. Polyak S. J., McArdle S., Liu S.-J. et al. Evolution of hepatitis С vims quasispecies in hypervariable region 1 and the putative interferon-sensitivity determining region during interferon therapy and natural infection // J. Virol. - 1998. - Vol. 72, N 5. - P. 4288-4296.
  14. Polyak S. J., Paschal D. M., McArdle S. et al. Characterization of the effects of hepatitis С virus nonstructural 5A protein expression in human cell lines and on interferon-sensitive virus replication // Hepatology. - 1999. - Vol. 29, N4. P. 1262-1271.
  15. Polyak S. J., Khabar K. S. A., Paschal D. M. A novel mechanism of hepatitis С Vims interferon resistence: induction of expression of the CXC hemokine, interleukin-8, by the non-structural 5A protein // European Cytokine Network (The official Journal of the European Cytokine Society). Special issue ICS/ ISICR. - 2000. - Vol. 11. - P. 215.
  16. Rady P. L., Cadet P., Bui Т. К. et al. Production of interferon gamma messenger RNA by cells of non-immune origin // Cytokine. - 1995. - Vol. 7, N 8. - P. 793-798.
  17. Taylor D. R., Shi S. Т., Lai M. M. С Hepatitis С vims and interferon resistance // Microb. and Infect. - 2000. - Vol. 2, N 2. - P. 1743-1756.-
  18. Thomas S. Harrison, Stuart M. Levitz. Role of 1L-12 in PBMC responses to fungi in persons with and without HIV infection // J. Immunol. - 1996. - Vol. 1, N 3. - P. 4492-4497.
  19. Yamamura M., Uyemura K., Deans R. J. et al. Defining protective responses to pathogens: cytokine profiles in leprosy lesions // Science. - 1991. - Vol. 254, N 11. - P. 277-279.

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Copyright (c) 2003 Narovlyansky A.N., Vershinina M.Y., Deryabin P.G., Mezentseva M.V., Ershov F.I.

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