An analysis of hepatitis-C virus proteins in the hepatic cells of patients with chronic hepatitis С


Cite item

Full Text

Abstract

A correlation between the detection of proteins and an activity of the pathological process was analyzed in a study of the content of the С virus hepatitis (CVH) proteins in hepatic cells of patients with chronic С hepatitis (CCH). The expression of CVH proteins in frozen sections of biopsy samples of 69 CCH patients was evaluated by using the immune-histological method involving original monoclonal antibodies (MCA) to 5 CVH proteins. The results of the detection of proteins in patients were compared with an activity and stage of CCH (by using histological tests and a level of alanine aminotransferase - AAT). A set of the CVH proteins were found in the liver of 74% of patients, i.e. core proteins, NS3, NS4A, NS4B and NS5A - in 28, 43,43, 55 and 58%, respectively. All studied proteins were detected in the cytoplasm of hepatocytes. Proteins were found in the liver more often as compared with the detection rate of CVH RNA in the blood serum (61%). This demonstrates a high sensitivity of the discussed test at detecting the CVH infection. The accumulation of the core protein was shown to correlate with the presence of the replicative form of CVH RNA in the liver and with a higher level of AAT. The quantity of NS5 A-expressing cells correlated directly with a CCH stage. The quantity of NSB- and NS3-positive hepatocytes correlated negatively with an activity of the infiammotary-and-necrotic processes in the liver. Hyper-fermentation was found more often among the antigen-positive patients. The CCH histological activity was proven to be reliably higher at a simultaneous detection of CCH proteins in the liver and of CVH RNA - in the serum.

References

  1. Абдулмеджидова А. Г., Масалова О. В., Атанадзе С. Н. и др. Характеристика панели моноклональных антител к рекомбинантному белку NS3 вируса гепатита С // Вопр. вирусол. - 2002. - № 1. - С. 21-25.
  2. Масалова О. В., Самохвалов Е. И., Петракова И. В. Выявление маркеров вируса гепатита С - белка нуклеокапсида, РНК и вирусспецифических антител в плазмах крови доноров // Там же. - 2000. - № 2. - С. 14-18.
  3. Масалова О. В., Абдулмеджидова А. Г., Атанадзе С. Н. и др. Характеристика панели моноклональных антител и эпитопное картирование белков вируса гепатита С // Докл. АН. - 2002. - Т. 383, № 4. - С. 545-550.
  4. Bartenschlager R., Lohmann V. Replication of hepatitis С virus // J. Gen. Virol. - 2000. - Vol. 81. - P. 1631-1648.
  5. Blight K., Rowland R., Hall P. et al. Immunohistochemical detection of the NS4 antigen of hepatitis С virus and its relation to histopathology // Am. J. Pathol. - 1993. - Vol. 143. - P. 1568-1573.
  6. Boyer N., Marcellin P. Pathogenesis, diagnosis and management of hepatitis С // J. Hepatol. - 2000. - Vol. 32. - P. 98-112.
  7. Desmet V. J., Gerber M., Hoofnagle J. H. et al. Classification of chronic hepatitis: diagnosis, grading and staging // Hepatology. 1994. - Vol. 19. - P. 1513-1520.
  8. Gonzalez-Peralta R. P., Fang J. W., Davis G. L. et al. Optimization for the detection of hepatitis С virus antigens in the liver // J. Hepatol. - 1994. - Vol. 20. - P. 143-147.
  9. Hahn C., Cho Y., Kang B. et al. The HCV core protein acts as a positive regulator of Fas-mediated apoptosis in a human Iimphoblastoid T-cells line // Virology. - 2000. - Vol. 276. - P. 127-137.
  10. Hiramatsu N., Hayashi N., Hurana Y. et al. Immunohistochemical detection of hepatitis С virus-infected hepatocytes in chronic liver disease with monoclonal antibodies to core, envelope and NS3 regions of the hepatitis С virus genome // Hepatology. - 1992. - Vol. 16. - P. 306-311.
  11. Lanford R. E., Sureau C., Jacob J. R. et al. Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis С virus using strand-specific RT- PCR // Virology. - 1994. - Vol. 202. - P. 604-610.
  12. Masalova O. V., Atanadze S. N., Samokhvalov E. I. et al. Detection of hepatitis С virus core protein circulating within different virus particle population // J. Med. Virol. - 1998. - Vol. 55. - P. 1-6.
  13. Negro F., Krawczynski K., Quadri R. et al. Detection of genomic- and minus-strand of hepatitis С virus RNA in the liver of chronic hepatitis С patients by strand-specific semiquantitative reverse-transcriptase polymerase chain reaction // Hepatology. - 1999. - Vol. 29. - P. 536-542.
  14. Nolandt O., Kern V., Muller H. et al. Analysis of hepatitis С virus core protein interaction domains // J. Gen. Virol. - 1997. - Vol.78. - P. 1331-1340.
  15. Okamoto H., Okada S., Sugiyama Y. Detection of hepatitis С virus RNA by a two-stage polymerase chain reaction with two pairs of praimers deduced from the 5'-noncoding region // Jpn. J. Exp. Med. - 1990. - Vol. 60. - P. 215-222.
  16. Ruggieri A., Harada Т., Matscuera Y., Miyamura T. Sensitization to Fas-mediated apoptosis by hepatitis С virus core protein // Virology. - 1997. - Vol. 229. - P. 68-76.
  17. Sansonno D., Cornacchiulo V., Racanelli V., Damacco F. In situ simultaneous detection of hepatitis С virus RNA and hepatitis С virus-related antigens in hepatocellular carcinoma // Cancer. - 1997. - Vol. 80. - P. 22-33.
  18. Shi S. Т., Polyak S. J., Tu H. et al. Hepatitis С virus NS5A collocatizes with the core protein on lipid droplets and interacts with apolipoproteins // Virology. - 2002. - Vol. 292. - P. 198-210.
  19. Tsutsumi M., Urashima S., Takada A. et al. Detection of antigens related to hepatitis С virus RNA encoding the NS5 region in the livers of patients with chronic type С hepatitis // Hepatology. - 1994. - Vol. 19. - P. 773-778.
  20. Yap S.-H., Willems M., Van den Oord J. et al. Detection of hepatitis С virus antigen by immunohistochemical staining: a histological marker of hepatitis С virus infection // J. Hepatol. - 1994. - Vol. 20. - P. 275-281.
  21. Yasui K., Wakita Т., Tsukiyama-Kohara K. et al. The native form and maturation process of hepatitis С virus core protein // J. Virol. - 1998. - Vol. 72. - P. 6048-6055.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2003 Masalova O.V., Abdulmedzhidova A.G., Shkurko T.V., Kelli E.I., Atanadze S.N., Zavalishina L.E., Frank G.A., Kuzina O.V., Lvov D.K., Kushch A.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies