Email this article ADAPTATION OF THE CORN EARWORM SINGLE NUCLEOCAPCIDE NUCLEOPOLYHEDROVIRUS (HELICOVERPA ZEA SNPV) FOR THE CONTROL OF THE COTTON BOLLWORM (HELICOVERPA ARMIGERA) POPULATION
- Authors: Kolosov A.V.1, Ternovoy V.A.1, Shvalov A.N.1, Moiseeva A.A.1, Safatov A.S.1, Mikheev V.N.1
-
Affiliations:
- State Research Center of Virology and Biotechnology «Vector»
- Issue: Vol 62, No 3 (2017)
- Pages: 134-137
- Section: ORIGINAL RESEARCH
- URL: https://virusjour.crie.ru/jour/article/view/155
- DOI: https://doi.org/10.18821/0507-4088-2017-62-3-134-137
- ID: 155
Cite item
Full Text
Abstract
Helicoverpa zea (Boddie, 1850) (Hz) single nucleocapcide nucleopolyhedrovirus (SNPV) was adapted to the cotton bollworm (Helicoverpa armigera, (Hübner, 1805) (Ha)) by five blind passages on larvae. The full genomic sequence of the resulting strain HS-18 has been determined (GenBank acc. №: KJ004000.1). Biological activity of the HS-18 strain is higher than the activity of all other Russian strains of NPV, as far as cotton bollworm strain HearSNPV-G4. HS-18-infected caterpillars at the 3-rd and 4-th ages died much faster than those infected with HearSNPV-G4 strain. A major difference of HS-18 genome is an 18 bp repeat in the RING-finger ORF that confirms high variability of this region. Three other insertions and seven base substitutions were observed earlier, while six base substitutions are new. Mutations are located at ORF42, lef-9, ORF58, VP39, PIF-4, P48, SOD, ORF111, ORF129 and ORF138 genes. Among all nucleotide mutation only one is synonymous. Thus we suppose the selective pressure to the virus. The resulting strain HS-18 is recommended as a biopesticide for controlling the number of cotton bollworm in cotton fields.
About the authors
A. V. Kolosov
State Research Center of Virology and Biotechnology «Vector»
Author for correspondence.
Email: kolosov_av@vector.nsc.ru
Russian Federation
V. A. Ternovoy
State Research Center of Virology and Biotechnology «Vector»
Email: noemail@neicon.ru
Russian Federation
A. N. Shvalov
State Research Center of Virology and Biotechnology «Vector»
Email: noemail@neicon.ru
Russian Federation
A. A. Moiseeva
State Research Center of Virology and Biotechnology «Vector»
Email: noemail@neicon.ru
Russian Federation
A. S. Safatov
State Research Center of Virology and Biotechnology «Vector»
Email: noemail@neicon.ru
Russian Federation
V. N. Mikheev
State Research Center of Virology and Biotechnology «Vector»
Email: noemail@neicon.ru
Russian Federation
References
- Ardisson-Araújo D.M.P., Sosa-Gomez D.R., Melo F.L., Bao S.N., Ribeiro B.M. Characterization of Helicoverpa zea single nucleopolyhedrovirus isolated in Brazil during the first old world bollworm (Noctuidae: Helicoverpa armigera) nationwide outbreak. Virus Reviews & Research. 2015; 20: 1=-4.
- Blissard G.W., Rohrmann G.F. Baculovirus diversity and molecular biology. Annu. Rev. Entomol. 1990; 35: 127-55.
- Jacques R.P. Persistence, accumulation and denaturation of Nuclear polyhedrosis and granulosis viruses. In: Summers M.D., Engler R., Falcon L.A., Vail P.V., eds. Baculoviruses for insect pest control: safety considerations. Washington, DC: American Society of Microbiology; 1975: 90-9.
- Management Gmb. Available at: http://slrecord.typepad.com/the_second_life_record/2015/01/management-gmb.html (accessed 14 July 2016).
- Andermatt Biocontrol: Products. Available at: http://www.export.biocontrol.ch/sites/products.html (accessed 20 June 2016).
- USDA Forest Service - Forest Health Protection Pesticide Viral Products. Available at: http://www.fs.fed.us/foresthealth/pesticide/virusproducts.shtml (accessed 22 June 2016).
- Pest Management Queensland: ViVus Max. Available at: http://www.agbitech.com/au/home/products/vivus-max.aspx (accessed 4 July 2016).
- Bio-Pesticides Introduction: Agri Life. Available at: http://www.agrilife.in/biopesticides.htm (accessed 23 June 2016).
- Chen X., Zhang W.J., Wong J., Chun G., Lu A., McCutchen B.F. et al. Comparative analysis of the complete genome sequences of Helicoverpa zea and Helicoverpa armigera single-nucleocapsid nucleopolyhedroviruses. J. Gen. Virol. 2002; 83(Pt 3): 673-84.
- Ignoffo C.M. The nuclear-polyhedrosis virus of Heliothis zea (boddie) and Heliothis virescens (fabricius). J. Invertebr. Pathol. 1965; 7(3): 315-19.
- Ferrelli M.L., Taibo C., Fichetti P., Sciocco-Cap A., Arneodo J.D. Characterization of a new Helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host, Helicoverpa gelotopoeon (Lepidoptera: Noctuidae). J. Invertebr. Pathol. 2016; 138: 89-93.
- Кошелев Ю.А., Рыжиков Г.А., Репин В.Е., Колосов А.В. Штамм вируса ядерного полиэдроза хлопковой совки Helicoverpa armigera Hbn., используемый для получения инсектицидного препарата. Патент РФ № 2359031; 2007.
- Божко Н.А., Горбунова Е.Е., Петрова И.Д., Караваев В.С., Колосов А.В. Штамм вируса ядерного полиэдроза капустной совки, используемый для получения инсектицидов. Патент РФ № 2153258; 1999.
- Okonechnikov K., Golosova O., Fursov M., UGENE team. Unipro UGENE: a unified bioinformatics toolkit. Bioinformatics. 2012; 28(8): 1166-67.
- Katoh K., Standley D.M. MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. Mol. Biol. Evol. 2013; 30(4): 772-80.
- Guindon S., Dufayard J.-F., Lefort V., Anisimova M., Hordijk W., Gascuel O. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst. Biol. 2010; 59(3): 307-21.
- Tamura K., Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 1993; 10(3): 512-26.
- Arrizubieta M., Williams T., Caballero P., Simón O. Selection of a nucleopolyhedrovirus isolate from Helicoverpa armigera as the basis for a biological insecticide. Pest Manag. Sci. 2014; 70(6): 967-76.
- Le T.H., Wu T., Robertson A., Bulach D., Cowan P., Goodge K. et al. Genetically variable triplet repeats in a RING-finger ORF of Helicoverpa species baculoviruses. Virus Res. 1997; 49(1): 67-77.
Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).