Problems of VirologyProblems of VirologyВопросы вирусологии0507-40882411-2097Central Research Institute for Epidemiology65910.36233/0507-4088-154ORIGINAL RESEARCHESОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯResearch ArticleThe incidence of infection in tumor and eye fluid system, and specific humoral immunity to herpes viruses in patients with uveal melanomaЧастота инфицированности опухоли, жидкостных сред глаза и специфический гуморальный иммунитет к герпесвирусам у пациентов с увеальной меланомойhttps://orcid.org/0000-0001-8920-2458SvetlovaE. V.СветловаЕ. В.
Russian Federation

virologist doctor of the Department of Immunology and Virology

врач-вирусолог вирусологической микробиологической лаборатории отдела иммунологии и вирусологии

qr888@ya.ruhttps://orcid.org/0000-0001-8007-6643BalatskayaN. V.БалацкаяН. В.
Russian Federation

PhD in Biological sciences, Head of the Department of Immunology and Virology

к.б.н., начальник отдела иммунологии и вирусологии

qr888@ya.ruhttps://orcid.org/0000-0001-8591-428XSaakyanS. V.СаакянС. В.
Russian Federation

Grand PhD in Medical sciences, Professor, Head of the Department of Ophthalmooncology and Radiology

д.м.н., профессор, начальник отдела офтальмоонкологии и радиологии

qr888@ya.ruhttps://orcid.org/0000-0003-1103-6570ZharovA. A.ЖаровА. А.
Russian Federation

researcher of the Department of Pathological Anatomy and Histology

научный сотрудник отдела патологической анатомии и гистологии

qr888@ya.ruhttps://orcid.org/0000-0001-7052-3294KrichevskayaG. I.КричевскаяГ. И.
Russian Federation

PhD in Medical sciences, leading researcher of the Department of Immunology and Virology

к.м.н., врач клинической лабораторной диагностики вирусологической микробиологической лаборатории отдела иммунологии и вирусологии

qr888@ya.ruhttps://orcid.org/0000-0001-7830-1880SvirinaI. V.СвиринаИ. В.
Russian Federation

PhD student of the Department of Ophthalmooncology and Radiology

аспирант отдела офтальмоонкологии и радиологии

qr888@ya.ruhttps://orcid.org/0000-0002-4713-5661IzmailovaN. S.ИзмайловаН. С.
Russian Federation

PhD in Medical sciences, Head of the Department of Pathological Anatomy and Histology

к.м.н., начальник отдела патологической анатомии и гистологии

qr888@ya.ruhttps://orcid.org/0000-0002-2087-7155MyakoshinaE. B.МякошинаЕ. Б.
Russian Federation

Grand PhD in Medical sciences, senior researcher of the Department of Ophthalmooncology and Radiology

д.м.н., научный сотрудник отдела офтальмоонкологии и радиологии

qr888@ya.ruHelmholtz Scientific Medical Research Center for Ophthalmic DiseasesФГБУ «Научный медицинский исследовательский центр глазных болезней им. Гельмгольца» Минздрава России11032023681374420122022Copyright ©; 2023, Svetlova E.V., Balatskaya N.V., Saakyan S.V., Zharov A.A., Krichevskaya G.I., Svirina I.V., Izmailova N.S., Myakoshina E.B.Copyright ©; 2023, Светлова Е.В., Балацкая Н.В., Саакян С.В., Жаров А.А., Кричевская Г.И., Свирина И.В., Измайлова Н.С., Мякошина Е.Б.2023Svetlova E.V., Balatskaya N.V., Saakyan S.V., Zharov A.A., Krichevskaya G.I., Svirina I.V., Izmailova N.S., Myakoshina E.B.Светлова Е.В., Балацкая Н.В., Саакян С.В., Жаров А.А., Кричевская Г.И., Свирина И.В., Измайлова Н.С., Мякошина Е.Б.https://creativecommons.org/licenses/by/4.0https://virusjour.crie.ru/jour/article/view/659

Introduction. Studies aimed at a direct research of human herpes viruses (HHVs) in the tumor material and eye media have not been carried out so far.

Research goal – to establish the frequency of detection HHVs DNA in the biomaterial of the eye and blood and to assess the specific humoral immunity to the causative agents of herpes virus infections in patients with uveal melanoma.

Materials and methods. 38 patients with the uveal tract tumor were examined for the presence of DNA of HHV types 1 and 2 (HSV-1, 2), Cytomegalovirus (CMV), Varicella Zoster virus (VZV), Epstein–Barr virus (EBV) and herpes viruses 6 and 8 types (HHV-6, HHV-8) in tumor tissue, vitreous body, aqueous humour and blood plasma by real-time polymerase chain reaction; blood serum was studied by enzyme-linked immunosorbent assay (ELISA) for IgG and IgM antibodies to HHVs.

Results. EBV DNA was present in tumor tissue in 20.6% of cases, in vitreous body in 4.2%, in blood plasma in 2.7%, and was not found in aqueous humor. Ig G antibodies to HSV-1, 2 and CMV were detected in 97.3% of cases, VZV – 94.6%, HHV-6 – 32.4%, antibodies to HHV-8 were not detected. 20 patients (55.6%) had reactivation of chronic HSV-1, 2 infection, and 14 (38.9%) patients had reactivation of CMV infection. Markers of chronic EBV infection were found in all patients, its atypical reactivation was observed in 2 cases (5.4%).

Conclusion. Our findings suggest the possible participation of EBV in the oncogenesis of the uveal tract and emphasize the need for further in-depth study of this problem.

Введение. До настоящего времени исследования, направленные на прямой поиск вирусов группы герпеса (ВГГ) в материале опухоли и средах глаза, не проводились.

Цель работы – установить частоту выявления ДНК ВГГ в биоматериале глаз, крови и провести оценку специфического гуморального иммунитета к возбудителям герпесвирусных инфекций у пациентов с увеальной меланомой (УМ).

Материалы и методы. Обследованы 38 пациентов с опухолями увеального тракта на наличие ДНК Herpes simplex virus (HSV-1, HSV-2), Cytomegalovirus (CMV), Varicella Zoster virus (VZV), virus Epstein–Barr (EBV), Human herpesvirus (HHV) 6 и HHV-8 в материале опухоли, стекловидного тела (СТ), влаги передней камеры (ВПК) и плазмы крови (ПК) методом полимеразной цепной реакции в реальном времени (ПЦР-РВ); пробы сыворотки крови исследованы методом иммуноферментного анализа (ИФА) на IgG-, IgM-антитела к ВГГ.

Результаты. ДНК EBV присутствовала в ткани опухоли в 20,6% случаев, в СТ – 4,2%, в ПК – 2,7%, во ВПК – не обнаружена. IgG-антитела к HSV-1, HSV-2 и CMV обнаружены в 97,3% случаев, VZV – 94,6%, HHV-6 – 32,4%, HHV-8 не выявлены. Реактивация хронической инфекции HSV-1, HSV-2 выявлена у 20 обследуемых (55,6%), CMV – у 14 (38,9%). Маркеры хронического инфицирования EBV обнаружены у всех пациентов, атипичной реактивации – в двух случаях (5,4%).

Заключение. Полученные данные позволяют говорить об участии EBV в развитии опухолей увеального тракта и необходимости дальнейшего углублённого исследования данной проблемы.

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