Problems of VirologyProblems of Virology0507-40882411-2097Central Research Institute for Epidemiology41010.36233/0507-4088-2020-65-4-228-236Study of the antiviral activity of the liquid corneal storage medium in relation to the herpes simplex virus <i>in vitro</i>AlimbarovaL. M.<p class="MsoNormal"><span lang="EN-US">Lyudmila M. Alimbarova – Cand. Med. Sci., assistant professor, leading researcher.</span></p><p class="MsoNormal">Moscow, 123098</p>virology@mail.ruhttps://orcid.org/0000-0002-8972-3111KerimovT. Z.<p class="MsoNormal"><span lang="EN-US">Timur Z. Kerimov – postgraduate of the Eye Disease Department.</span></p><p class="MsoNormal"><span lang="EN-US">Moscow, 127473</span></p>fake@neicon.ruhttps://orcid.org/0000-0001-8967-6370BorzenokS. A.<p class="MsoNormal"><span lang="EN-US">Sergei A. Borzenok – Dr. Med. Sci., Acad. of RANS, Professor of the Eye Disease Department, A.I. Yevdokimov Moscow State University of Medicine and Dentistry of the Ministry of Health; Head of the Center for Fundamental and Applied Biomedical Problems of S.N. Fedorov Eye Microsurgery Federal State Institution of the Ministry of Health.</span></p><p class="MsoNormal"><span lang="EN-US">Moscow, 127486</span></p>fake@neicon.ruhttps://orcid.org/0000-0001-9160-6240National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. GamaleyaA.I. Evdokimov Moscow State University of Medicine and DentistryS. Fyodorov Eye Microsurgery Complex Federal State Institution170920206542282361709202017092020Copyright © 2020, Alimbarova L.M., Kerimov T.Z., Borzenok S.A.2020<p class="MsoNormal"><strong><span lang="EN-US">The aim</span></strong><span lang="EN-US"> of the study was to assess the antiviral activity of the 7 types of liquid corneal storage medium on an experimental model of herpesvirus infection in vitro.</span></p><p class="MsoNormal"><strong><span lang="EN-US">Material and methods.</span></strong><span lang="EN-US"> The study of antiviral activity of 7 samples of liquid corneal storage medium on a model of herpesvirus infection caused by the herpes simplex virus type 1 in Vero-cell using virological and statistical research methods was carried out.</span></p><p class="MsoNormal"><strong><span lang="EN-US">Results and discussion</span></strong><span lang="EN-US">. All the studied images of the corneal storage medium, including the Borzenka-Moroz base medium, did not have a cytotoxic effect on Vero cell culture. Out of of 7 samples of liquid corneal storage medium, 4 samples had reliable antiviral activity against HSV-1 when used under the therapeutic regimen (1 hour after infection) and under the preventive regimen (2 hours before infection). Antiviral activity was established in 2 samples containing the interferon inducer cycloferon at a concentration of 10 mg/kg and 30 mg/kg (sample 2, 3), in a sample containing the interferon inducer gamapren 15 mg/kg (sample 5), and in a sample containing a combination of drugs - 10 mg/kg cycloferon and an acyclic nucleoside analog-acyclovir 10 mg/kg (sample 6). According to the results of 2 test regimens, the maximum statistically significant inhibitory effect in relation to HSV-1 was detected in sample 6, containing a combination of drugs. Against the background of sample 6, the infectious activity of the test virus decreased by an average of 3.2 lg, the inhibition coefficient was 54.5%.</span></p><p class="MsoNormal"><strong><span lang="EN-US">Conclusion.</span></strong><span lang="EN-US"> The results of the study indicate the prospects of using types of media with antiviral activity (samples 2, 3, 5, 6) for storing donor corneas in order to increase the effectiveness of keratoplasty in patients with ophthalmic herpes.</span></p>herpes simplex virus type 1HSV-1herpesvirus infectionliquid corneal storage mediumcornea preservationantiviral activityвирус простого герпеса 1-го типаофтальмогерпесгерпесвирусная инфекцияжидкая среда для хранения роговицыконсервация роговицыпротивовирусная активность[1. Исаков В.А., ред. Герпесвирусные инфекции человека: руководство для врачей. СПб.: СпецЛит; 2013.][2. Resnikoff S., Pascolini D., Etya’ale D., Kocur I., Pararajasegaram R., Pokharel G.P., et al. 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