Problems of VirologyProblems of VirologyВопросы вирусологии0507-40882411-2097Central Research Institute for Epidemiology38510.36233/0507-4088-2020-65-3-126-131REVIEWSОБЗОРЫRETRACTED: High-throughput sequencing in diagnostics and prevention of herpes simplex virus infection (Herpesviridae, Alphaherpesvirinae, Simplexvirus, Human alphaherpesvirus 1)СТАТЬЯ ОТОЗВАНА: Высокопродуктивное секвенирование в диагностике и профилактике инфекции простого герпеса (Herpesviridae, Alphaherpesvirinae, Simplexvirus, Human alphaherpesvirus 1)https://orcid.org/0000-0003-3407-2224SergeyevO. V.СергеевО. В.
Russian Federation

Oleg V. Sergeyev, Doct. Sci. (Biol.), Professor at the Department of microbiology, virology and immunology

Moscow, 119991

Сергеев Олег Витальевич, д-р биол. наук, проф. каф. микробиологии, вирусологиии иммунологии 

119991, Москва

osergeyev123@gmail.comhttps://orcid.org/0000-0003-4789-4964Bosh’ianR. E.БошьянР. Е.
Russian Federation

Roman E. Bosh’ian

Moscow, 119991

119991, Москва

https://orcid.org/0000-0002-9909-3598BarinskyI. F.БаринскийИ. Ф.
Russian Federation

Igor F. Barinsky

Moscow, 123098

123098, Москва

I.M. Sechenov First Moscow State Medical University (Sechenov University)ФГАОУ ВО Первый Московский государственный медицинский университет имени И.М. Сеченова Минздрава России (Сеченовский Университет)National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. GamaleyaФГБУ «Национальный исследовательский центр эпидемиологии и микробиологии имени почётного академика Н.Ф. Гамалеи» Минздрава России220720206531261312107202021072020Copyright ©; 2020, Sergeyev O.V., Bosh’ian R.E., Barinsky I.F.Copyright ©; 2020, Сергеев О.В., Бошьян Р.Е., Баринский И.Ф.2020Sergeyev O.V., Bosh’ian R.E., Barinsky I.F.Сергеев О.В., Бошьян Р.Е., Баринский И.Ф.https://creativecommons.org/licenses/by/4.0https://virusjour.crie.ru/jour/article/view/385

RETRACTED

Herpes simplex viruses types 1 (HSV-1) and 2 (HSV-2) are among the most common viruses in the human population. The clinical manifestations of HSV infection vary widely, which necessitates reliable molecular methods for the timely diagnosis of herpes virus infection, as well as for detection of mutations in the genes responsible for drug resistance. PCR is often unable to detect HSV isolates with nucleotide substitutions at the primer binding site. Sanger sequencing of the whole genome reveals mutations mainly at the consensus level, which accumulate at advanced stages of viral infection. High-throughput sequencing (HTS, next generation sequencing) offers an obvious advantage both in early diagnosis of herpes virus infection and identification of HSV variants.

СТАТЬЯ ОТОЗВАНА

Вирусы простого герпеса (ВПГ) 1-го (ВПГ-1) и 2-го (ВПГ-2) типа относятся к числу наиболее распространённых в человеческой популяции. Клинические проявления простого герпеса широко варьируют, что обусловливает необходимость разработки надёжных молекулярных методов для своевременной диагностики герпесвирусной инфекции, а также для обнаружения мутаций в генах, отвечающих за лекарственную устойчивость. Применение ПЦР часто неспособно идентифицировать изоляты ВПГ с заменами нуклеотидов в участке связывания праймеров. Полногеномное секвенирование по Сэнгеру выявляет мутации в основном на консенсусном уровне, они накапливаются уже на продвинутой стадии вирусной инфекции. Высокопродуктивное секвенирование (секвенирование следующего поколения) имеет явные преимущества как в ранней диагностике герпесвирусной инфекции, так и в идентификации вариантов ВПГ.

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