Problems of VirologyProblems of Virology0507-40882411-2097Central Research Institute for Epidemiology378Development of the disease in marmot at the intranasal infection with the monkeypox virusSergeevA. A.<p>Sergeev Aleksandr, research assistant</p><p>630559, Koltsovo, Novosibirsk Region</p>sergeev_ala@vector.nsc.ruKabanovA. S.630559, Koltsovo, Novosibirsk Regionfake@neicon.ruBulychevL. E.630559, Koltsovo, Novosibirsk Regionfake@neicon.ruSergeevAr. A.630559, Koltsovo, Novosibirsk Regionfake@neicon.ruPyankovO. V.630559, Koltsovo, Novosibirsk Regionfake@neicon.ruBodnevS. A.630559, Koltsovo, Novosibirsk Regionfake@neicon.ruGalakhovaD. O.630559, Koltsovo, Novosibirsk Regionfake@neicon.ruZamedyanskayaA. S.630559, Koltsovo, Novosibirsk Regionfake@neicon.ruTitovaK. A.630559, Koltsovo, Novosibirsk Regionfake@neicon.ruShishkinaL. N.630559, Koltsovo, Novosibirsk Regionfake@neicon.ruAgafonovA. P.630559, Koltsovo, Novosibirsk Regionfake@neicon.ruSergeevA. N.630559, Koltsovo, Novosibirsk Regionfake@neicon.ruState Research Center for Virology and Biotechnology “Vector”2812201560637411207202012072020Copyright © 2015, Sergeev A.A., Kabanov A.S., Bulychev L.E., Sergeev A.A., Pyankov O.V., Bodnev S.A., Galakhova D.O., Zamedyanskaya A.S., Titova K.A., Shishkina L.N., Agafonov A.P., Sergeev A.N.2015<span>In experimental study the sensitivity of the Marmota bobak species to the monkeypox virus (MPXV) with the intranasal (i/n) infection was tested. It was demonstrated that 50% of the infective dose (ID 50) of the MPXV on external clinical signs of the disease was 2.2 lg plaque forming units (PFU). The percentage of the marmot mortality is slightly dependent on the infecting dose of the MPXV, therefore it is not possible to correctly determine the value of 50 % fatal dose (FD50) for these animals. The most pronounced external clinical signs of the disease were obtained in the marmots: pox-like skin rash throughout the surface of the body and mucous membranes, purulent discharge from the nose, lymphadenitis, discoordination, tremor of the extremities, fever, increased aggression, and ruffled fur. In the course of experiments intended to determine the dynamics of the accumulation of the MPXV in various organs, tissues, and blood serum of marmot infected i/n with dose of 3.7 lg PFU, it was found that the trachea, lungs, and the bifurcation lymph nodes are the primary target organs. The trachea, lungs, nasal mucosa membrane, and skin are the organs with maximal virus replication recorded at 5, 7, 9, and 12 days after the infection. The transfer of the MPXV into the secondary target organs (nasal mucosa membrane, brain, spleen, duodenum, adrenal glands, and skin) was carried out in marmots with lymphogenic and hematogenic ways of the dissemination of the infection.</span>monkeypox virusmarmotintranasal infectionsusceptibilityclinical signs of diseasedynamics of accumulationdisseminationвирус оспы обезьянсурокинтраназальное заражениечувствительностьклинические признаки заболеваниядинамика накоплениядиссеминация[1. Hutson C.L., Carroll D.S., Self J., Weiss S., Hughes C.M., Braden Z. et al. Dosage comparison of Congo Basin and West African strains of monkeypox virus using a prairie dog animal model of systemic orthopoxvirus disease. Virology. 2010; 402 (1): 72–82.][2. Zaucha G., Jahrling P., Geisbert T., Swearengen J., Hensley L. The pathology of experimental aerosolized monkeypox virus infection in cynomolgus monkeys (macaca fascicularis). Lab. Invest. 2001; 81: 1581–600.][3. 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