Problems of VirologyProblems of Virology0507-40882411-2097Central Research Institute for Epidemiology324Infectious, immunogenic, and protective characteristics of the Rift Valley fever virus depending on the passage level and storage conditionsZakutskiyN. I.Nikolay Zakutsky, Doctor of Veterinary Scienceszaknikiv43@yandex.ruBalyshevaV. I.fake@neicon.ruKhukhorovaI. Ju.fake@neicon.ruYurkovS. G.fake@neicon.ru2804201560241431007202010072020Copyright © 2015, Zakutskiy N.I., Balysheva V.I., Khukhorova I.J., Yurkov S.G.2015<span>The report discusses the research into the impact of some factors, especially the passage in a suspension of continuous cells BHK-21/13 and storage at different temperatures, upon immunobiological characteristics of the Rift Valley fever (RVF) virus strain 1974-VNIIVViM. The limits for the passage levels and optimal storage conditions providing maximal infectious and immunogenic activity, as well as protection of the attenuated RVF strain 1974-VNIIVViM, were determined. It was found that the RVF virus growth in VHK-21/23 cell suspension in the course of 20 consecutive passages and storage at -50°C for 1 to 2 years did not reduce any infectious, immunogenic or protective characteristics of the virus. It was also shown that the RVF virus strain 1974-VNIIVViM could be stored at the following temperature ranges: 1 month at 4 to 6°C, 4 months at -10 to -12 °C, 6 months at -20 °C, and up to 2 years at -50 °C.</span>Rift Valley fever (RVF)viruspassage levelstorage temperatureinfectivityimmunogenicityprotectionлихорадка долины Рифтвирусуровень пассажейтемпература храненияинфекционностьиммуногенностьпротективность[1. Балышев В.М., Жуков А.Н., Грачев Д.В., Саттаров И.Т., Болгова М.В. Аттенуированный штамм вируса оспы коз. Патент РФ № 2325437, 1996.][2. Закутский Н.И. Разработка и совершенствование технологии изготовления инактивированной вакцины против инфекционного ринотрахеита крупного рогатого скота: Дисс. … д-ра вет. наук. Покров; 1998.][3. Колян Ф.И. Моделирование иммунного ответа и анализ факторов эпизоотического процесса: Автореф. дисс. … канд. биол. наук. Щелково; 2012.][4. Румянцева Е.А., Балышев В.М., Жуков А.Н., Ногина И.В., Сафонов Г.А. Аттенуированный штамм вируса болезни Найроби. Патент РФ № 2348692, 2006.][5. Сергеев В.А. Культивирование и репродукция вирусов животных. М.: Колос; 1976.][6. Pepin M., Bouloy M., Bird B.H., Kemp A., Paweska J. Rift Valley fever virus (Bunyavi ridae : Plebovirus): an update on pathogenesis, molecular epidemiology, vectors, diagnocstics and prevention. Vet. Res. 2010; 41(6): 61.]