Problems of VirologyProblems of Virology0507-40882411-2097Central Research Institute for Epidemiology25410.36233/0507-4088-2019-64-6-274-280Belgorod region – the territory endemic for hepatitis EKyuregyanK. K.<p><span class="fontstyle0">Dr. Sci. Biol., Chief Researcher of the Research Institute of Molecular and Personalized Medicine, Lead Researcher of laboratory of viral hepatitis </span></p><p><br />Moscow, 105064</p><p>Moscow, 125993</p>karen-kyuregyan@yandex.ruhttps://orcid.org/0000-0002-3599-117XPolyakovA. D.<p>Moscow, 143026</p><p>Belgorod, 308015</p><p> </p>fake@neicon.ruhttps://orcid.org/0000-0002-7140-2897PotemkinI. A.<p>Moscow, 105064</p><p>Moscow, 125993</p>fake@neicon.ruhttps://orcid.org/0000-0001-7559-4219KarlsenA. A.<p>Moscow, 105064</p><p>Moscow, 125993</p>fake@neicon.ruhttps://orcid.org/0000-0002-6013-7768IsaevaO. V.<p>Moscow, 105064</p><p>Moscow, 125993</p>fake@neicon.ruhttps://orcid.org/0000-0002-2656-3667LopatukhinaM. A.Moscow, 105064fake@neicon.ruhttps://orcid.org/0000-0001-6853-4154MullinE. V.Moscow, 105064fake@neicon.ruSlukinovaO. S.Moscow, 105064fake@neicon.ruMalinnikovaE. Yu.<p>Moscow, 105064</p><p>Moscow, 125993</p>fake@neicon.ruhttps://orcid.org/0000-0002-5501-5707ShibrikE. V.Belgorod, 308005fake@neicon.ruOgleznevaE. E.<p>Belgorod, 308015</p><p>Belgorod, 308023</p>fake@neicon.ruMikhailovM. I.<p>Moscow, 105064</p><p>Moscow, 125993</p>fake@neicon.ruhttps://orcid.org/0000-0002-6636-6801Mechnikov Research Institute for Vaccines and SeraRussian Medical Academy of Continuous Professional EducationSkolkovo Territorial Division of the Office of Rospotrebnadzor for the City of Moscow RussiaBelgorod State National Research UniversityDepartment of Health and Social Protection of the Population of Belgorod RegionBelgorod Regional Department of Rospotrebnadzor201220196462742800302202003022020Copyright © 2019, Kyuregyan K.K., Polyakov A.D., Potemkin I.A., Karlsen A.A., Isaeva O.V., Lopatukhina M.A., Mullin E.V., Slukinova O.S., Malinnikova E.Y., Shibrik E.V., Oglezneva E.E., Mikhailov M.I.2019<span class="fontstyle0"><strong>Introduction</strong>. </span><span class="fontstyle2">Belgorod region is the territory with the highest incidence of hepatitis E in the Russian Federation.<br /></span><strong><span class="fontstyle0">The aim </span></strong><span class="fontstyle2"><strong>of the study</strong> was to comprehensively characterize the circulation of hepatitis E virus (HEV) in the Belgorod region, including the study of population immunity to the virus, determining the prevalence of infection among the pig population and analysis of the genetic diversity of HEV from patients and animals.<br /></span><span class="fontstyle0"><strong>Material and methods</strong>. </span><span class="fontstyle2">Serum samples of a conditionally healthy population (</span><span class="fontstyle3">n </span><span class="fontstyle2">= 2027) of all age groups were tested for anti-HEV IgG and IgM by ELISA with commercial assays. HEV RNA was determined in fecal samples from pigs aged 2–4 months </span><span class="fontstyle3">(n </span><span class="fontstyle2">= 526), in sewage samples from pig farms (</span><span class="fontstyle3">n </span><span class="fontstyle2">= 10), as well as in stool samples from patients with hepatitis E (</span><span class="fontstyle3">n </span><span class="fontstyle2">= 6) using reverse transcription polymerase chain reaction (RT-PCR). Phylogenetic analysis was performed for an amplified 300 nt fragment corresponding to HEV open reading frame 2.<br /></span><span class="fontstyle0"><strong>Results and discussion</strong>. </span><span class="fontstyle2">The prevalence of anti-HEV IgG in general population averaged 16.4% (95% CI: 14.8–18.1; 332/2027). The proportion of individuals who had both anti-HEV IgM and IgG averaged 2.8% (95% CI: 2.2–3.6; 57/2027). The incidence rate of anti-HEV IgG increased with age, from 2.8% (95% CI: 1.3-5.8) in children aged 1–14 years to 40.1% (95% CI: 34.9-45.6) in people 70 years or older. The detection rate of HEV RNA in pigs was 20% (95% CI: 16.8-23.6; 105/526). HEV RNA was detected in 2 out of 10 sewage samples. The HEV sequences isolated from patients with hepatitis E, pigs, and sewage samples in Belgorod region belonged to the HEV genotype 3, had a 95-100% homology, and formed common clusters on a phylogenetic tree.<br /></span><strong><span class="fontstyle0">Conclusions. </span></strong><span class="fontstyle2">The high prevalence of HEV in pigs population has led to the formation of an endemic territory in the Belgorod region, which is the center of pig breeding. Measures aimed at reducing the circulation of HEV among pig population and decontamination of sewage from pig farms are necessary to control HEV infection.</span>hepatitis Ehepatitis E viruszoonosisгепатит Евирус гепатита Езооноз[1. 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