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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:ali="http://www.niso.org/schemas/ali/1.0/" article-type="research-article" dtd-version="1.2" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">Problems of Virology</journal-id><journal-title-group><journal-title xml:lang="en">Problems of Virology</journal-title><trans-title-group xml:lang="ru"><trans-title>Вопросы вирусологии</trans-title></trans-title-group></journal-title-group><issn publication-format="print">0507-4088</issn><issn publication-format="electronic">2411-2097</issn><publisher><publisher-name xml:lang="en">Central Research Institute for Epidemiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">12267</article-id><article-categories><subj-group subj-group-type="toc-heading" xml:lang="en"><subject>Articles</subject></subj-group><subj-group subj-group-type="toc-heading" xml:lang="ru"><subject>Статьи</subject></subj-group><subj-group subj-group-type="article-type"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title xml:lang="en">Molecular-genetic characterization of the Okhotskiy virus (OKHV) and Aniva virus (ANIV) (Orbivirus, Reoviridae) isolated from the ticks Ixodes (Ceratixodes) uriae White, 1852 in High Latitudes of the Northern Eurasia</article-title><trans-title-group xml:lang="ru"><trans-title>Молекулярно-генетическая характеристика вирусов Охотский (OKHV-Okhotskiy virus) и Анива (ANIV-Aniva virus) (Reoviridae, Orbivirus), изолированных в высоких широтах Северной Евразии из облигатных эктопаразитов чистиковых птиц (Alcidae Leach, 1820) - клещей Ixodes (Ceratixodes) uriae White, 1852</trans-title></trans-title-group></title-group><pub-date date-type="pub" iso-8601-date="2014-04-15" publication-format="electronic"><day>15</day><month>04</month><year>2014</year></pub-date><volume>59</volume><issue>2</issue><issue-title xml:lang="en">VOL 59, NO2 (2014)</issue-title><issue-title xml:lang="ru">ТОМ 59, №2 (2014)</issue-title><fpage>22</fpage><lpage>28</lpage><history><date date-type="received" iso-8601-date="2023-06-09"><day>09</day><month>06</month><year>2023</year></date></history><permissions><copyright-statement xml:lang="en">Copyright ©; 2014, Lvov D.K., Alkhovsky S.V., Shchelkanov M.Y., Shchetinin A.M., Deryabin P.G., Gitelman A.K., Samokhvalov E.I., Botikov A.G.</copyright-statement><copyright-statement xml:lang="ru">Copyright ©; 2014, Львов Д.К., Альховский С.В., Щелканов М.Ю., Щетинин А.М., Дерябин П.Г., Гительман А.К., Самохвалов Е.И., Ботиков А.Г.</copyright-statement><copyright-year>2014</copyright-year><copyright-holder xml:lang="en">Lvov D.K., Alkhovsky S.V., Shchelkanov M.Y., Shchetinin A.M., Deryabin P.G., Gitelman A.K., Samokhvalov E.I., Botikov A.G.</copyright-holder><copyright-holder xml:lang="ru">Львов Д.К., Альховский С.В., Щелканов М.Ю., Щетинин А.М., Дерябин П.Г., Гительман А.К., Самохвалов Е.И., Ботиков А.Г.</copyright-holder><ali:free_to_read xmlns:ali="http://www.niso.org/schemas/ali/1.0/"/><license><ali:license_ref xmlns:ali="http://www.niso.org/schemas/ali/1.0/">https://creativecommons.org/licenses/by/4.0</ali:license_ref></license></permissions><self-uri xlink:href="https://virusjour.crie.ru/jour/article/view/12267">https://virusjour.crie.ru/jour/article/view/12267</self-uri><abstract xml:lang="en"><p>Molecular-genetic characteristics of the Okhotskiy virus (OKHV) and Aniva virus (ANiV) were studied (iD GenBank KF981623-32). These viruses are distributed over the shelf and island areas in the high latitudes in the Okhotsk, Bering, and Barents seas and linked with nesting colonies of Alcidae seabirds and their obligatory parasites, the Ixodes uriae (Ixodidae) ticks. OKHV and ANiV are observed to be independent species within the limits of the Great island virus (GiV) group of the Orbivirus genus of the Reoviridae family. The majority of the genes of OKHV and ANiV have high homology (VP1 - 96%, Т2 - 99%, VP7 (T13) - 98%, NS1 - 94%, NS2 - 98%, NS3 - 72%, VP6 - 93%). Nevertheless, the envelope proteins containing the main specific antigenic determinants (VP2 and VP5) of OKHV and ANiV are sufficiently different (62% and 68% homology for amino acid sequences, respectively).</p></abstract><trans-abstract xml:lang="ru"><p>Изучены молекулярно-генетические характеристики вирусов Охотский (OKHV - Okhotskiy virus) (iD Gen Bank KF981623-32) и Анива (ANiV - Aniva virus), ареал распространения которых охватывает в высоких широтах шельфовые и островные территории бассейнов Охотского, Берингова и Баренцева морей. Вирусы экологически связаны с гнездовьями чистиковых (Alcidae) птиц и их облигатными паразитами клещами Ixodes uriae (Ixodidae). OKHV и ANiV являются самостоятельными видами в составе группы Грейт-Айленд (GiV - Great island virus) рода Orbivirus сем. Reoviridae. Большинство генов OKHV и ANiV имеют между собой высокий уровень гомологии (VP1 96%; Т2 99%; VP7 (T13) 98%; NS1 94%; NS2 98%; NS3 72%; VP6 93%). Но поверхностные белки, несущие основные видоспецифические антигенные детерминанты (VP2 и VP5), у OKHV и ANiV существенно различаются (62 и 68% гомологии по аминокислотным последовательностям соответственно).</p></trans-abstract><kwd-group xml:lang="en"><kwd>Reoviridae</kwd><kwd>Orbivirus</kwd><kwd>OKHV</kwd><kwd>ANIV</kwd><kwd>KEMV</kwd><kwd>GIV</kwd><kwd>Alcidae</kwd><kwd>Ixodidae</kwd><kwd>Ixodes (Ceratixodes) uriae</kwd><kwd>Reoviridae</kwd><kwd>Orbivirus</kwd><kwd>Okhotskiy virus (OKHV)</kwd><kwd>Aniva virus (ANIV)</kwd><kwd>Kemerovo virus (KEMV)</kwd><kwd>Great Island virus (GIV)</kwd><kwd>high latitudes</kwd><kwd>colonial seabirds</kwd><kwd>Alcidae</kwd><kwd>Ixodidae</kwd><kwd>Ixodes (Ceratixodes) uriae</kwd><kwd>Okhotsk sea</kwd><kwd>metagenomic analysis</kwd></kwd-group><kwd-group xml:lang="ru"><kwd>вирус Охотский</kwd><kwd>вирус Анива</kwd><kwd>вирус Кемерово</kwd><kwd>вирус Грейт-Айленд</kwd><kwd>высокие широты</kwd><kwd>колониальные морские птицы</kwd><kwd>Охотское море</kwd><kwd>метагеномный анализ</kwd></kwd-group></article-meta></front><body></body><back><ref-list><ref id="B1"><label>1.</label><mixed-citation>Lvov D.K., Timofeeva A.A., Gromashevski V.L., Tsyrkin Y.M., Veselovskaya O.V., Gostinshchikova G.V et al. “Okhotskiy” virus, a new arbovirus of the Kemerovo group isolated from Ixodes (Ceratix-odes) putus Pick.-Camb. 1878 in the Far East. 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