Problems of VirologyProblems of VirologyВопросы вирусологии0507-40882411-2097Central Research Institute for Epidemiology12150ArticlesСтатьиResearch ArticlePeculiarity of the Laboratory Diagnostic of the Measles Virus Infection in Previously Vaccinated and Unvaccinated PatientsОсобенности лабораторной диагностики кори у больных с разным прививочным анамнезом15102012575VOL 57, NO5 (2012)ТОМ 57, №5 (2012)212609062023Copyright ©; 2012, Mamaeva T.A., Lipskaya G.Y., Naumova M.A., Shulga S.V., Mulders M., Featherstone D.A., Zavyalova L.A., Chernyshova E.V., Zamyatina E.P., Kuznetsova N.N.Copyright ©; 2012, Мамаева Т.А., Липская Г.Ю., Наумова М.А., Шульга С.В., Mulders M., Featherstone D.A., Завьялова Л.А., Чернышова Е.В., Замятина Е.П., Кузнецова Н.Н.2012Mamaeva T.A., Lipskaya G.Y., Naumova M.A., Shulga S.V., Mulders M., Featherstone D.A., Zavyalova L.A., Chernyshova E.V., Zamyatina E.P., Kuznetsova N.N.Мамаева Т.А., Липская Г.Ю., Наумова М.А., Шульга С.В., Mulders M., Featherstone D.A., Завьялова Л.А., Чернышова Е.В., Замятина Е.П., Кузнецова Н.Н.https://creativecommons.org/licenses/by/4.0https://virusjour.crie.ru/jour/article/view/12150

119 specimens of blood sera collected from measles cases with different vaccination history (aged 4 months to 48 years) on 5th-6th days after rash onset were investigated using EIA. The obtained results showed that the primary immune response (PIR) was developed in 59 patients; the secondary immune response (SIR) was developed in 60 patients with a significant increase in the specific high avidity IgG (22.34 IU/ml ± 3.2). The specific IgM were detected in 100% cases studied with capture ELISA in both previously vaccinated and unvaccinated individuals of different age. The specific IgM were detected by indirect ELISA in 100% cases in unvaccinated patients, while IgM positive sera was defined only in 23.3% of individuals with SIR. It was concluded that measles virus infection in previously vaccinated and unvaccinated adults had clinical differences. The role of patients with SIR in virus transmission was discussed.

Методом ИФА были исследованы 119 сывороток, полученных на 5-6-й день после появления сыпи от больных корью (в возрасте 4 мес-48 лет) с разным вакцинальным анамнезом. Результаты показали, что встреча с вирусом кори у 59 пациентов была первичной, у 60 больных отмечен вторичный иммунный ответ со значительным увеличением специфических высокоавидных IgG (22,34 ± 3,2 МЕ/мл). Установлено, что тесты capture-формата позволяют выявлять IgM в 100% случаев, независимо от прививочного анамнеза больного. Положительный (100%) результат в тесте indirect-формата был получен только при исследовании сывороток непривитых больных, тогда как при вторичном иммунном ответе IgM были определены в 23,3% случаев. Клинические различия были выявлены у непривитых и привитых взрослых лиц. Обсуждается роль больных с вторичным иммунным ответом в трансмиссии вируса.

IgMIgGmeaslesELISAthe primary and secondary immune responseIgM IgGavidity IgGкорьИФАпервичный и вторичный иммунный ответавидность IgGАшмарин И. П., Воробьев А. А. Статистические методы в микробиологических исследованиях. Л.; 1962.Зверев В. В., Маркушин С. Г., Юминова Н. В. Корь. СПб.;- 2004.Тихонова Н. Т., Алешкин В. А., Герасимова А. Г. и др. теоретические и практические аспекты элиминации кори в России. В кн.: теоретические и практические аспекты элиминации кори в России: Сборник науч. трудов. М., 2005: С.14-18.Arista S., Ferraro D., Cascio A. et al. Detection of IgM antibodies specific for measles virus by capture and indirect enzyme immunoassays. Res. Virol. 1995; 146 (3): 225-232.Center for Disease Control. Case definitions for public health surveillance. Morbid. Mortal. Weekly Rep. 1990; 39: 13.Chen R. T., Markowitz L. E., Albrect P. et al. Measles antibody: reevaluation of protective titers. J. Infect. Dis. 1990; 162: 1036-1042.Damien B., Huiss S., Sheider F., Muller C.P. Estimated susceptibility to asimptomatic secondary immune response against measles in late convalescent and vaccinated persons. J. Med.Virol. 1998; 56: 85-90.Hickman C.J., Hyde T. B., Sovers S. B. et al. Laboratory characterization of measles virus infection in previously vaccinated and unvaccinated individuals. J. Infect. Dis. 2011. 204 (suppl. 1): 549-558.Moss W. J., Strebel P. M. Biological of measles eradication. J. Infect. Dis. 2011; 204: 47-53.Muller C. P. Measles elimination: old and new challenges? Vaccine. 2001; 19 (17-19): 2258-2261.Onishchenko G., Ezhlova E., Gerasimova A. et al. Progress toward measles elimination in the Russian Federation, 2003-2009. J. Infect. Dis. 2011; 20: 366-372.Ratnam S., Tipples G., Head C. et al. Perfomance of indirect immunoglobulin M (IgM) serology test and IgM capture assays for laboratory diagnosis of measles. J. Clin. Microbiol. 2000; 38: 99-104.Rota J. S., Hickman C. J., Sovers S. B. et al. Two case studies of modified measles in vaccinated physicians exposed to primary measles case: high risk of infection but low risk of transmission. J. Infect. Dis. 2011; 204: 559-563.Samoilovich E. O., Yermalovich М. A., Semeiko G. V. et al. Outbreak of measles in Belarus, January-June 2006. Euro Surveill. 2006; 11 (30): 3011.Shulga S. V., Rota P.A., Kremer J. R. et al. Genetic variability of wildtype measles viruses, circulating in the Russian Federation during the implementation of the National Measles Elimination Program, 2003-2007. Clin. Microbiol. Infect. 2009; 15 (6):.528-537.Siedler A., Mankertz A., Feil F. et al. Closer to the goal: Effors in measles elimination in Germany 2010. J. Infect. Dis. 2011; 204: 373-380.Strebel P.M., Cochi S. L., Hoekstra E. et al. A world without measles. J. Infect. Dis. 2011; 204: Р.1-3.Tikhonova N. T., Bichurina M. A., Gerasimova A. G. et al. Enhanced surveillance for measles in low-icidence territories of the Russian Federation: defining a rate for suspected case investigation. Epidemiol. Infect.2011; 139: 239-246.Velicko I., Muller L. L., Pebody R. et al. Nationwide measles epidemic in Ukraine: the effect of low vaccine effectiveness. Vaccine. 2008; 26: 6980-6985.WHO - World Health Organization. Manual for the laboratory diagnosis of measles and rubella virus infection. 2-nd ed. Geneva. Switzerland: WHO; 2006:.22.http://www.euro.who.int/en/what-we-do/health-topics/disease-prevention/ vaccines-and-immunization/publications/who-epidemiological-briefs/who-epidemiological-brief-18-measles-outbreaks,-member-state-responses,-measles-exportation-to-the-americas-region,-afp-surveillance-and-the-polio-outbreak-in-China