Problems of VirologyProblems of VirologyВопросы вирусологии0507-40882411-2097Central Research Institute for Epidemiology12078ArticlesСтатьиPhylogenetic analysis of cytomegaloviruses isolated from man and different primate speciesФилогенетический анализ цитомегаловирусов, выделенных от людей и разных видов приматов15042011562NO2 (2011)№2 (2011)283209062023Copyright ©; 2011, Agumava A.A., Chikobava M.G., Lapin B.A., Tonkonozhenko O.A., Pavlovskiy A.N., Agumava A.A., Chikobava M.G., Lapin B.A., Tonkonozhenko O.A., Pavlovsky A.N.Copyright ©; 2011, Агумава А.А., Чикобава М.Г., Лапин Б.А., Тонконоженко О.А., Павловский А.Н., Agumava A.A., Chikobava M.G., Lapin B.A., Tonkonozhenko O.A., Pavlovsky A.N.2011Agumava A.A., Chikobava M.G., Lapin B.A., Tonkonozhenko O.A., Pavlovskiy A.N., Agumava A.A., Chikobava M.G., Lapin B.A., Tonkonozhenko O.A., Pavlovsky A.N.Агумава А.А., Чикобава М.Г., Лапин Б.А., Тонконоженко О.А., Павловский А.Н., Agumava A.A., Chikobava M.G., Lapin B.A., Tonkonozhenko O.A., Pavlovsky A.N.https://creativecommons.org/licenses/by/4.0https://virusjour.crie.ru/jour/article/view/12078

The conserved regions of nucleotide sequences were found in primate cytomegaloviruses (CMV). Universal primers were designed for the consensus sequence of a conservative region of the UL56 gene of the betaherpesvirinae subfamily. Amplification, sequencing, and phylogenetic analysis of the fragments of CMV strains isolated from man and different primate species were made. Analysis of sequenced gene fragments showed that the UL56 gene area is most suitable for the phylogenetic analysis of primate CMV and could identify several groups of clusters by the degree of relationship among the viruses of this family.

Выявлены консервативные регионы нуклеотидных последовательностей цитомегаловирусов (ЦМВ) приматов. Разработаны универсальные праймеры к консенсусной последовательности консервативного участка гена UL56 подсемейства betaherpesvirinae. Проведены амплификация, секвенирование и филогенетический анализ фрагментов ЦМВ, выделенных от людей и обезьян разных видов. Анализ секвенированных фрагментов генов показал, что участок гена UL56 является наиболее подходящим для филогенетического анализа ЦМВ приматов, и позволил выделить несколько групп кластеров по степени родства среди вирусов данного семейства.

cytomegalovirusprimatesphylogenetic analysisцитомегаловирусприматыфилогенетический анализBale J. F., Petheram S. J., Souza I. E., Murph J. R. Cytomegalovirus reinfection in young children // J. Pediatr. - 1996. - Vol. 128. - P. 347-352.Bogner E., Reschke M., Reis B. et al. Identification of the gene product encoded by ORF UL56 of the human cytomegalovirus genome // Virology. - 1993. - Vol. 196. - P. 290-293.Bruggeman C., Mejer H., Dormans P. et al. Isolation of a cytomegalovirus-like agent from wild rats // Arch. Virol. - 1982. - Vol. 73. - P. 231-241.Eizuru Y., Tsuchiya K., Mori R., Minamishima Y. Immunological and molecular comparison of simian cytomegaloviruses isolated from African green monkey (Cercopithecus aethiops) and Japanese macaque (Macaca fuscata) // Arch. Virol. - 1989. - Vol. 107. - P. 65-75.Francis N., Boylston A., Roberts A. et al. Cytomegalovirus infection in gastrointestinal tracts of patients infected with HIV-1 or AIDS // J. Clin. Pathol. - 1989. - Vol. 42. - P. 1055-1064.Gonczol E., Danczig E., Boldogh I. et al. In vivo model for the acute, latent and reactivated phases of cytomegalovirus infection // Acta Microbiol. - 1985. - Vol. 32. - P. 39-47.Griffiths P. D., Cope A. V., Hassan-Walker A. F., Emery V. C. Diagnostic approaches to cytomegalovirus infection in bone marrow and organ transplantation // Transplant. Infect. Dis. - 1999. - Vol. 1. - P. 179-186.Harkins L., Volk A. L., Samanta M. et al. Specific localisation of human cytomegalovirus nucleic acids and proteins in human colorectal cancer // Lancet. - 2002. - Vol. 360. - P. 1557- 1563.Harwood A. J. Basic DNA and RNA protocols Introduction // Meth. Mol. Biol. - 1996. - Vol. 58. - P. 447-451.Holland G., Tufail A., Jordon M. Cytomegalovirus diseases // Ocular infection and immunity / Eds J. S. Pepose et al. - St. Louis, 1996. - P. 1088-1128.Huang E. S., Alford C. A., Reynolds D. W. et al. Molecular epidemiology of cytomegalovirus infections in women and their infants // N. Engl. J. Med. - 1980. - Vol. 303. - P. 958-962.Kinney J. S., Onorato I. M., Stewart J. A. et al. Cytomegaloviral infection and disease // J. Infect. Dis. - 1985. - Vol. 151. - P. 772-774.Koffron A. J., Patterson B. K., Yan S. et al. Latent human cytomegalovirus: a functional study // Transplant. Proc. - 1997. - Vol. 29. - P. 793-795.Michaels M. G., Alcendor D. J., Rinaldo C. R. et al. Distinguishing baboon cytomegalovirus from human cytomegalovirus: importance for xenotransplantation // J. Infect. Dis. - 1997. - Vol. 176. - P. 1476-1483.Nelson C. T., Demmler G. J. Cytomegalovirus infection in the pregnant mother, fetus and newborn infant // Clin. Perinatol. - 1997. - Vol. 1. - P. 151-160.Spector D. H., Spector S. A. The oncogenic potential of human cytomegalovirus // Prog. Med. Virol. - 1984. - Vol. 29. - P. 45-89.Yow M. D., Williamson D. W., Leeds L. J. et al. Epidemiologic characteristics of cytomegalovirus infection in mothers and their infants // Am. J. Obstet. Gynecol. - 1988. - Vol. 158. - P. 1189-1195.