Problems of VirologyProblems of VirologyВопросы вирусологии0507-40882411-2097Central Research Institute for Epidemiology11956ArticlesСтатьиThe capacities of current test systems to verify early HIV infectionВозможности современных тест-систем при подтверждении ранней ВИЧ-инфекции15102009545NO5 (2009)№5 (2009)374009062023Copyright ©; 2009, Baranova E.N., Sharipova I.N., Denisova N.M., Susekina M.A., Puzyrev V.F., Sarkisyan K.A., Vorobyeva M.S., Burkov A.N., Ulanova T.I.Copyright ©; 2009, Баранова Е.Н., Шарипова И.Н., Денисова Н.М., Сусекина М.Е., Пузырев В.Ф., Саркисян К.А., Воробьева М.С., Бурков А.Н., Уланова Т.И.2009Baranova E.N., Sharipova I.N., Denisova N.M., Susekina M.A., Puzyrev V.F., Sarkisyan K.A., Vorobyeva M.S., Burkov A.N., Ulanova T.I.Баранова Е.Н., Шарипова И.Н., Денисова Н.М., Сусекина М.Е., Пузырев В.Ф., Саркисян К.А., Воробьева М.С., Бурков А.Н., Уланова Т.И.https://creativecommons.org/licenses/by/4.0https://virusjour.crie.ru/jour/article/view/11956

The purpose of the present investigation was to comparatively evaluate the performance characteristics of the test systems designed to verify the positive results of screening survey for HIV infection, such as the solid-phase immunoassay DS-EIA-HIV-AB/AG-SPECTR (Diagnosticheskiye Sistemy (Diagnostic Systems) Research-and-Production Association, Nizhni Novgorod) and tests based on immune blotting (IB). The investigation examined 15 seroconversion panels produced by ZeptoMetrix (USA) and BBI (USA). The use of the DS-EIA-HIV-AB/AG-SPECTR test system determined 88 of the 167 seroconversion panels as HIV positive. The IB-based tests revealed only 45 of the 167 samples as positive. Consequently, the application of the DS-EIA-HIV-AB/AG-SPECTR test system is more effective than the IB-based tests in early HIV infection.

Цель настоящей работы - проведение сравнительной оценки рабочих характеристик тест-систем, предназначенных для подтверждения положительных результатов, полученных при скрининговых исследованиях на ВИЧ-инфекцию - твердофазного иммуноферментного теста "ДС-ИФА-ВИЧ-АТ/АГ-СПЕКТР 88" (НПО "Диагностические системы", Нижний Новгород) и тестов на основе метода иммунного блоттинга (ИБ). В работе были исследованы 15 сероконверсионных панелей производства компаний ZeptoMetrix (США) и BBI (США). При использовании тест-системы "ДС-ИФА-ВИЧ-АТ/АГ-СПЕКТР" из 167 образцов сероконверсионных панелей определяются как ВИЧ-позитивные. Тесты на основе ИБ выявляют лишь 45 из 167 образцов как позитивные. Следовательно, на стадии ранней ВИЧ-инфекции использование "ДС-ИФА-ВИЧ-АТ/АГ-СПЕКТР" более эффективно, чем тестов на основе ИБ.

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