Problems of VirologyProblems of VirologyВопросы вирусологии0507-40882411-2097Central Research Institute for Epidemiology11933ArticlesСтатьиMethods for diagnosis of prion diseasesМетоды диагностики прионных заболеваний15102009545NO5 (2009)№5 (2009)4909062023Copyright ©; 2009, Grigoriev V.B., Pokidyshev A.N., Kalnov S.L., Klimenko S.M.Copyright ©; 2009, Григорьев В.Б., Покидышев А.Н., Кальнов С.Л., Клименко С.М.2009Grigoriev V.B., Pokidyshev A.N., Kalnov S.L., Klimenko S.M.Григорьев В.Б., Покидышев А.Н., Кальнов С.Л., Клименко С.М.https://creativecommons.org/licenses/by/4.0https://virusjour.crie.ru/jour/article/view/11933

The epidemic of bovine spongiform encephalopathy (BSE) in the United Kingdom, its related occurrence of a new type of Creutzfeld-Jacob disease and proven cases of this type of the disease transmitted by blood transfusion initiated intensive studies to develop a inexpensive, prompt, and sensitive method for the early lifetime diagnosis of prion diseases. This would permit initiation of the timely treatment of the patients and prevention of contamination of foodstuffs. However, despite significant progress made in this direction, this objective has not yet been achieved. The present review highlights the currently available methods for the diagnosis of transmissible spongiform encephalopathies, as well as the latest developments in the ultrasensitive detection of these diseases, which is based on the misfolded prion protein complex.

Эпидемия губкообразной энцефалопатии крупного рогатого скота в Великобритании и связанное с ней возникновение среди людей нового варианта болезни Крейтцфельда-Якоба (нвБКЯ), а также доказанные случаи заражения пациентов нвБКЯ путем переливания крови инициировали интенсивные исследования в области создания недорогого, быстрого и чувствительного метода ранней прижизненной диагностики прионных заболеваний. Это позволило бы начать своевременное лечение пациентов и предотвратить попадание контаминированных продуктов в пищевую цепь. Однако, несмотря на значительный прогресс, достигнутый в этом направлении, поставленная задача полностью не была решена. В настоящем обзоре представлены существующая на сегодняшний день информация о методах диагностики трансмиссивных губкообразных энцефалопатий, а также последние разработки в области ультрачувствительной детекции этих заболеваний, основанной на изменении конформации белка приона.

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